Tubes containing fish tissues in transport medium for cell cultivation or RT-PCR/RT-qPCR analysis shall be placed in insulated containers, such as thick-walled polystyrene boxes, together with sufficient ice or an alternative cooling medium with the similar cooling effect to ensure chilling of the samples during transportation to the laboratory.

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Buisjes met visweefsels in transportmedium voor celkweek of RT-PCR/RT-qPCR-analyse worden in geïsoleerde recipiënten geplaatst, zoals dikwandige polystyreendozen, samen met voldoende ijs of een alternatief koelmiddel met een vergelijkbaar koelvermogen, zodat de monsters tijdens het vervoer naar het laboratorium koel worden gehouden.

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If evidence of CPE has been observed in a cell culture, medium (supernatant) shall be collected and examined by one or more of the following techniques: Enzyme-linked immunosorbent essay (ELISA), Immunofluorescence (IF), neutralisation, RT-PCR or RT-qPCR.

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Wanneer in een celcultuur CPE wordt geconstateerd, wordt het medium (supernatans) verzameld en onderzocht met behulp van één of meer van de volgende technieken: enzymgekoppelde immuunadsorbent-techniek (ELISA), immunofluorescentie (IF), neutralisatie, RT-PCR of RT-qPCR.

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virus detection by RT-PCR or RT-qPCR;

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virusdetectie met behulp van RT-PCR of RT-qPCR;

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Other RT-PCR or RT-qPCR versions of proven similar efficacy may be used instead.

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In plaats hiervan mogen ook andere RT-PCR- of RT-qPCR-varianten waarvan is aangetoond dat zij vergelijkbaar effectief zijn, worden gebruikt.

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virus isolation in cell cultures followed by identification using enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT), virus neutralisation test or real-time reverse transcriptase polymerase chain reaction (RT-qPCR); or

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virusisolatie in celculturen, gevolgd door identificatie met behulp van de enzymgekoppelde immuunadsorbent-techniek (enzyme-linked immunosorbent assay, ELISA), de indirecte immunofluorescentietest (indirect fluorescent antibody test, IFAT), de virusneutralisatietest of de realtime-reverse-transcriptase-polymerasekettingreactie (real-time reverse transcriptase polymerase chain reaction, RT-qPCR); ofwel

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Date index: 2021-10-16